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Recombinant Mouse IL-13 Protein

Recombinant Mouse Interleukin-13 is produced by our Mammalian expression system and the target gene encoding Pro22-Phe131 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Interleukin-13; IL-13; T-Cell Activation Protein P600; Il13; Il-13

UniProt

P20109

Expression Region

Pro22-Phe131

Tag

C-6His

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Recombinant Mouse IL-13RA2 (C-Fc) (Cat#orb2992724) at 2μg/ml (100 μl/well) can bind Recombinant Mouse IL-13 (110AA, C-6His) (Cat#orb3002385) .The ED50 of Recombinant Mouse IL-13 (110AA, C-6His) (Cat#orb3002385) is 10.98ng/ml. (Regularly tested) . Cell Based Assay: Measured in a cell proliferation assay using TF‐1 human erythroleukemic cells. The ED50 for this effect is 11.34 ng/ml. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 13.1 KDa. Observed: 14-30 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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