Recombinant Mouse Leptin Protein

Recombinant Mouse Leptin is produced by our E.coli expression system and the target gene encoding Val22-Cys167 is expressed.

Product Specifications

Product Name Alternative

Leptin; Obese Protein; Obesity Factor; LEP; OB; OBS

UniProt

Q544U0

Expression Region

Val22-Cys167

Tag

Tag free

Field of Research

Metabolism Research

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

BLI: Loaded Recombinant Mouse LEPR (C-10His) (Cat#orb2993840) on HIS1K Biosensor, can bind Recombinant Mouse Leptin (Cat#orb3002378) with an affinity constant of 0.196 nM as determined in BLI assay. (Regularly tested) . ELISA: Immobilized Recombinant Mouse LEPR (C-10His) (Cat#orb2993840) at 5μg/ml (100 μl/well) can bind Recombinant Mouse Leptin (Cat#orb3002378) *. *: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Mouse Leptin (Cat#orb3002378) is 1.16 ng/ml. (Regularly tested) .

Form

Lyophilized

Molecular Weight

Predicted: 16.1 KDa. Observed: 14 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.