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Recombinant Human NRG1Beta Protein

Recombinant Human Neuregulin-1 Beta is produced by our E.coli expression system and the target gene encoding Ser2-Lys246 is expressed.

Product Specifications

Product Name Alternative

Pro-neuregulin-1; Neuregulin-1 beta 1; NRG1-beta 1; HRG1-beta 1; EGF; NRG1; GGF; HGL; HRGA; NDF; SMDF; Heregulinβ-1

Expression Region

Ser2-Lys246

Tag

Tag free

Endotoxin

< 0.01 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 85% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 85% as determined by SEC-HPLC. (Regularly tested)

Bioactivity

Cell Based Assay: Human breast cancer organoids were cultured with Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002494), R-spondin 3 (Cat#orb2993001), KGF (Cat#orb3002467), FGF-10 (Cat#orb3002478), EGF (Cat#orb3002493) and NRG1-beta 1 (Cat#753) . DAPI (blue), Ki67 (red) and ER (green) .

Form

Lyophilized

Molecular Weight

Predicted: 26.9 KDa. Observed: 34 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

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