Recombinant Human IL-4 Protein
Recombinant Human Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His25-Ser153 is expressed with a human IgG1 Fc tag at the C-terminus.
Product Specifications
Product Name Alternative
Interleukin-4; IL-4; B-Cell Stimulatory Factor 1; BSF-1; Binetrakin; Lymphocyte Stimulatory Factor 1; Pitrakinra; IL4
UniProt
P05112
Expression Region
His25-Ser153
Tag
C-Fc
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Bioactivity
BLI: Loaded Cynomolgus IL-4RA-His (Cat#orb2994860) on HIS1K Biosensor, can bind Human IL-4-Fc (Cat#orb3002348) with an affinity constant of -3 nM as determined in BLI assay. Loaded Human IL-4RA-His (Cat#orb2994200) on HIS1K Biosensor, can bind Human IL-4-Fc (Cat#orb3002348) with an affinity constant of -3 nM as determined in BLI assay. . ELISA: Immobilized Human IL-4-Fc (Cat#orb3002348) at 5μg/ml (100 μl/well) can bind Cynomolgus IL-4Ra-His (Cat#orb2994860) .The ED50 of Recombinant Human IL-4Ra-His (Cat#orb2994860) is 79.49 ng/ml.
Form
Lyophilized
Molecular Weight
Predicted: 41.9 KDa. Observed: 40-55 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
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