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Recombinant Human IL-2 Protein

Recombinant Human Interleukin-2 is produced by our E.coli expression system and the target gene encoding Pro22-Thr153 (Cys145Ser) is expressed.

Product Specifications

Product Name Alternative

Interleukin-2; IL-2; T-cell growth factor; TCGF; Aldesleukin

UniProt

P60568

Expression Region

Pro22-Thr153 (Cys145Ser)

Tag

Tag free

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

BLI: Loaded Human IL-2RA-His (Cat#orb2994464) on HIS1K Biosensor, can bind Human IL-2 (Cat#orb3002347) with an affinity constant of 31 nM as determined in BLI assay. Loaded Human IL-2RA-Fc (Cat#orb2994463) on Protein A Biosensor, can bind Human IL-2 (Cat#orb3002347) with an affinity constant of 13.2 nM as determined in BLI assay. Loaded Human IL-2RB-His (Cat#orb2994462) on HIS1K Biosensor, can bind Human IL-2 (Cat#orb3002347) with an affinity constant of 0.19 uM as determined in BLI assay. Loaded Human IL-2RB-Fc (Cat#orb2994461) on Protein A Biosensor, can bind Human IL-2 (Cat#orb3002347) with an affinity constant of 0.27 uM as determined in BLI assay. Loaded Mouse IL-2RA-His (Cat#orb2994413) on HIS1K Biosensor, can bind Human IL-2 (Cat#orb3002347) with an affinity constant of 33 nM as determined in BLI assay. Loaded Biotinylated Human IL-2RB-His-Avi (Cat#orb2993868) on HIS1K Biosensor, can bind Human IL-2 (Cat#orb3002347) with an affinity constant of 0.34 uM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using CTLL‐2 mouse cytotoxic T cells. The ED50 for this effect is 90-270 pg/ml.

Form

Lyophilized

Molecular Weight

Predicted: 15.5 KDa. Observed: 14 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 10mM Acetata-Na, 5% Trehaiose, pH 4.5.

Available Sizes

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