Recombinant Human TNF alpha Protein
Recombinant Human Tumor Necrosis Factor Alpha is produced by our E.coli expression system and the target gene encoding Gly57-Leu233 is expressed with a 6His tag at the N-terminus.
Product Specifications
Product Name Alternative
Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2
UniProt
P01375
Expression Region
Gly57-Leu233
Tag
N-6His
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
BLI: Loaded Human TNF alpha-His (Cat#orb3002254) on HIS1K Biosensor, can bind Human TNF RII-mFc (Cat#orb2994890) with an affinity constant of 1.83 nM as determined in BLI assay. (Regularly tested) . Cell Based Assay: Measured in a cytotoxicity assay using L‐929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 30-150 pg/ml. (QC verified)
Form
Lyophilized
Molecular Weight
Predicted: 21.8 KDa. Observed: 18 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM Histidine, 8 %Trehalose, 0.05%Tween80, pH 5.0.
Available Sizes
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