Recombinant Human IL-2 Protein
Recombinant Human Interleukin-2 Superkine is produced by our Mammalian expression system and the target gene encoding Ala21-Thr153 (L100F, R101D, L105V, I106V, I112F) is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Interleukin-2; IL-2; T-Cell Growth Factor; TCGF; Aldesleukin; IL2
UniProt
P60568
Expression Region
Ala21-Thr153 (L100F, R101D, L105V, I106V, I112F)
Tag
C-6His
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
BLI: Loaded Human IL-2RA-Fc (Cat#orb2994463) on Protein A Biosensor, can bind Human IL-2 Superkine (Cat#orb3002229) with an affinity constant of 10.4 nM as determined in BLI assay. Loaded Human IL-2RB-Fc (Cat#orb2994461) on Protein A Biosensor, can bind Human IL-2 Superkine (Cat#orb3002229) with an affinity constant of 0.58 nM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using CTLL‐2 mouse cytotoxic T cells. The ED50 for this effect is 151.39 pg/ml.
Form
Lyophilized
Molecular Weight
Predicted: 16.5 KDa. Observed: 16&18 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 4mM HCl.
Available Sizes
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