Recombinant Mouse EGF Protein

Recombinant Mouse Epidermal Growth Factor is produced by our E.coli expression system and the target gene encoding Asn977-Arg1029 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Pro-epidermal growth factor; Epidermal growth factor; EGF

UniProt

P01132

Expression Region

Asn977-Arg1029

Tag

C-6His

Field of Research

Cancer Biology

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

Cell Based Assay: Measured by its ability to inhibits the proliferation of human epithelial A431 cells.The ED50 for this effect is 2-20 ng/ml (QC verified) . Human endometrial epithelial organoids were cultured with EGF (Cat#orb3002397) and R-spondin 1 (Cat#orb3002497) . The organoids showed good morphology. Data were kindly provided by Novoprotein's customer. Thanks for her contribution.

Form

Lyophilized

Molecular Weight

Predicted: 7.2 KDa. Observed: 9-14 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.