Recombinant Human IL-17A Protein

ELISA: Immobilized Human IL-17RA-Fc (Cat#orb2994535) at 2μg/ml (100 μl/well) can bind Human IL-17A-His (Cat#orb2994915) .The ED50 of Human IL-17A-His (Cat#orb2994915) is 0.49 ng/ml. Immobilized Human IL-17RA-His (Cat#orb2994076) at 2μg/ml (100 μl/well) can bind Human IL-17A-His (Cat#orb2994915) .*: Biotinylated by NHS-biotin prior to testing.The ED50 of Human IL-17A-His (Cat#orb2994915) is 7.39 ng/ml.. BLI: Loaded Human IL-17A Antibody (9E6) (Cat#DA055) on Pro-A Biosensor, can bind Recombinant Human IL-17A (C-6His) (Cat#orb2994915) with an affinity constant of 12.7 pM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human IL-17RA-Fc (Cat#orb2993676) on Pro A Biosensor, can bind Human IL-17A-His (Cat#orb2994915) with an affinity constant of 0.003 nM as determined in BLI assay.. Cell Based Assay: Measured by its ability to induce IL-6 secretion by NIH‐3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 1-10 ng/ml.

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.