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Recombinant Human TNF RII Protein

Recombinant Human Tumor Necrosis Factor Receptor Superfamily Member 1B is produced by our Mammalian expression system and the target gene encoding Pro24-Thr206 is expressed with a mouse IgG1 Fc tag at the C-terminus.

Product Specifications

Product Name Alternative

Tumor necrosis factor receptor superfamily member 1B; TNFRSF1B; Tumor necrosis factor receptor 2; TNF-R2; TNF-RII; Tumor necrosis factor receptor type II; p75; p80 TNF-alpha receptor; CD120b

UniProt

P20333

Expression Region

Pro24-Thr206

Conjugation

Unconjugated

Tag

C-mFc

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Recombinant Human TNF RII-mFc (Cat#orb2994890) at 1μg/ml (100 μl/well) can bind Recombinant Human TNF alpha-His (Cat#orb3002254) . The ED50 of Recombinant Human TNF alpha-His (Cat#orb3002254) is 1.97 ng/ml. (Regularly tested) . BLI: Loaded Human TNF alpha-His (Cat#orb3002254) on HIS1K Biosensor, can bind Human TNF RII-mFc (Cat#orb2994890) with an affinity constant of 1.83 nM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 46.44 KDa. Observed: 60 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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