Recombinant Cynomolgus IL-4RA Protein
Recombinant Cynomolgus Interleukin-4 Receptor Subunit Alpha is produced by our Mammalian expression system and the target gene encoding Met26-Arg232 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Interleukin-4 receptor subunit alpha; IL-4R-alpha; CD124; IL4-BP; Soluble IL-4R-alpha
UniProt
G7Q0S7
Expression Region
Met26-Arg232
Conjugation
Unconjugated
Tag
C-6His
Origin
Cynomolgus
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human IL-4-Fc (Cat#orb3002348) at 5μg/ml (100 μl/well) can bind Cynomolgus IL-4Ra-His (Cat#orb2994860) .The ED50 of Recombinant Human IL-4Ra-His (Cat#orb2994860) is 79.49 ng/ml.. BLI: Loaded Human IL-4-Fc (Cat#orb3002348) on AHC Biosensor, can bind Cynomolgus IL-4RA-His (Cat#orb2994860) with an affinity constant of 0.48 nM as determined in BLI assay. . Cell Based Assay: Measured by its ability to inhibit IL-4-dependent proliferation of TF‐1 human erythroleukemic cells.The ED50 for this effect is 2.38ng/ml.
Form
Lyophilized
Molecular Weight
Predicted: 24.6 KDa. Observed: 38-58 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
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