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Recombinant Cynomolgus IL-4RA Protein

Recombinant Cynomolgus Interleukin-4 Receptor Subunit Alpha is produced by our Mammalian expression system and the target gene encoding Met26-Arg232 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Interleukin-4 receptor subunit alpha; IL-4R-alpha; CD124; IL4-BP; Soluble IL-4R-alpha

UniProt

G7Q0S7

Expression Region

Met26-Arg232

Conjugation

Unconjugated

Tag

C-6His

Origin

Cynomolgus

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Human IL-4-Fc (Cat#orb3002348) at 5μg/ml (100 μl/well) can bind Cynomolgus IL-4Ra-His (Cat#orb2994860) .The ED50 of Recombinant Human IL-4Ra-His (Cat#orb2994860) is 79.49 ng/ml.. BLI: Loaded Human IL-4-Fc (Cat#orb3002348) on AHC Biosensor, can bind Cynomolgus IL-4RA-His (Cat#orb2994860) with an affinity constant of 0.48 nM as determined in BLI assay. . Cell Based Assay: Measured by its ability to inhibit IL-4-dependent proliferation of TF‐1 human erythroleukemic cells.The ED50 for this effect is 2.38ng/ml.

Form

Lyophilized

Molecular Weight

Predicted: 24.6 KDa. Observed: 38-58 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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