Recombinant Cynomolgus PD-L1 Protein
Recombinant Cynomolgus CD274 Molecule is produced by our Mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a human IgG1 Fc tag at the C-terminus.
Product Specifications
Product Name Alternative
B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1
UniProt
G7PSE7
Expression Region
Phe19-Thr239
Conjugation
Unconjugated
Tag
C-Fc
Origin
Cynomolgus
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Cynomolgus PD-L1-Fc (Cat#orb2994783) at 2μg/ml (100 μl/well) can bind Anti-Human PDL1 mAb (Cat#orb2993144) .The ED50 of Anti-Human PDL1 mAb (Cat#orb2993144) is 8.15 ng/ml.. BLI: Loaded Biotinylated Human PD-1-His-Avi (Cat#orb2993936) on SA Biosensor, can bind Cynomolgus PD-L1-Fc (Cat#orb2994783) with an affinity constant of 14.1 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 52.4 KDa. Observed: 73 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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