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Recombinant Cynomolgus PD-L1 Protein

Recombinant Cynomolgus CD274 Molecule is produced by our Mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a human IgG1 Fc tag at the C-terminus.

Product Specifications

Product Name Alternative

B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1

UniProt

G7PSE7

Expression Region

Phe19-Thr239

Conjugation

Unconjugated

Tag

C-Fc

Origin

Cynomolgus

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Cynomolgus PD-L1-Fc (Cat#orb2994783) at 2μg/ml (100 μl/well) can bind Anti-Human PDL1 mAb (Cat#orb2993144) .The ED50 of Anti-Human PDL1 mAb (Cat#orb2993144) is 8.15 ng/ml.. BLI: Loaded Biotinylated Human PD-1-His-Avi (Cat#orb2993936) on SA Biosensor, can bind Cynomolgus PD-L1-Fc (Cat#orb2994783) with an affinity constant of 14.1 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 52.4 KDa. Observed: 73 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

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