Recombinant Human Siglec-2 Protein
Recombinant Human B-cell Receptor CD22 is produced by our Mammalian expression system and the target gene encoding Asp20-Arg687 is expressed with a human IgG1 Fc tag at the C-terminus.
Product Specifications
Product Name Alternative
B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigenMGC130020; CD22 molecule; CD22; sialic acid binding Ig-like lectin 2; Siglec-2; SIGLEC2FLJ22814; T-cell surface antigen Leu-14
UniProt
P20273
Expression Region
Asp20-Arg687
Conjugation
Unconjugated
Tag
C-Fc
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Anti-Human Siglec-2 mAb (Cat#orb2993148) at 2μg/ml (100 μl/well) can bind Human Siglec-2-Fc (Cat#orb2994728) .The ED50 of Human Siglec-2-Fc (Cat#orb2994728) is 24.05 ng/ml.
Form
Lyophilized
Molecular Weight
Predicted: 102.3 KDa. Observed: 120-150 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM Tris-HCl, 1mM EDTA, 150mM NaCl, pH 8.0 .
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