Recombinant Human uPAR Protein
Recombinant Human Urokinase Plasminogen Activator Surface Receptor is produced by our Mammalian expression system and the target gene encoding Leu23-Arg303 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Urokinase Plasminogen Activator Surface Receptor; U-PAR; uPAR; Monocyte activation antigen Mo3; CD87; PLAUR; MO3; UPAR
UniProt
Q03405
Expression Region
Leu23-Arg303
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human uPAR-His (Cat#orb2995110) at 2μg/ml (100 μl/well) can bind Human uPA-His* (Cat#orb2993548) .*: Biotinylated by NHS-biotin prior to testing. The ED50 of Human uPA-His (Cat#orb2993548) is 7.19 ng/ml. (Regularly tested) Immobilized Recombinant Human uPAR (C-6His) (Cat#orb2995110) at 2μg/ml (100 μl/well) can bind Anti-Human uPAR Antibody (Mouse IgG1) .The ED50 of Anti-Human uPAR Antibody (Mouse IgG1) is 5.26ng/ml. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 32.4 KDa. Observed: 52 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM Tris-HCl, 10% Trehalose, 2% Mannitol, 0.05% Tween 80, pH 8.0.
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