Recombinant Human /Cynomolgus CD28 Protein
Recombinant Human T-cell-specific Surface Glycoprotein CD28 is produced by our Mammalian expression system and the target gene encoding Asn19-Pro152 is expressed with a 6His tag at the N-terminus.
Product Specifications
Product Name Alternative
CD28; CD28 antigen; CD28 molecule; T-cell-specific surface glycoprotein CD28; Tp44; TP44
UniProt
P10747
Expression Region
Asn19-Pro152
Conjugation
Unconjugated
Tag
N-6His
Origin
Human/Cynomolgus
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human/Cynomolgus CD28-His (Cat#orb2995095) at 2μg/ml (100 μl/well) can bind Anti-CD28 mAb (Cat#GMP-A063) The ED50 of Anti-CD28 mAb (Cat#GMP-A063) is 15.3 ng/ml. (Regularly tested) . BLI: Loaded Anti-Human CD28 mAb-Fc (Cat#GMP-A063) on Protein A Biosensor, can bind Human/Cynomolgus CD28-His (Cat#orb2995095) with an affinity constant of 0.77 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 16.7 KDa. Observed: 30-40 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
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