Recombinant Human Siglec-3 Protein
Recombinant Human Sialic Acid Binding Ig-like Lectin 3 is produced by our Mammalian expression system and the target gene encoding Asp18-His259 is expressed with a human IgG1 Fc, 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Myeloid Cell Surface Antigen CD33; Sialic Acid-Binding Ig-Like Lectin 3; Siglec-3; gp67; CD33; SIGLEC3
Expression Region
Asp18-His259
Conjugation
Unconjugated
Tag
C-Fc-6His
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Recombinant Human Siglec-3 (C-Fc-6His) (Cat#orb2995073) at 1μg/ml (100 μl/well) can bind Anti-Human CD33 Antibody (Gemtuzumab ozogamicin) *. *: Biotinylated by NHS-biotin prior to testing.The EC50 of Anti-Human CD33 Antibody (Gemtuzumab ozogamicin) is 4.95ng/ml. (Regularly tested) .
Form
Lyophilized
Molecular Weight
Predicted: 54.9 KDa. Observed: 80 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 150mM NaCl, 2mM EDTA, pH 7.2.
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