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Recombinant Human Nectin-1 Protein

Recombinant Human Poliovirus Receptor-Related Protein 1 is produced by our Mammalian expression system and the target gene encoding Gln31-Thr334 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Poliovirus Receptor-Related Protein 1; Herpes Virus Entry Mediator C; Herpesvirus Entry Mediator C; HveC; Herpesvirus Ig-Like Receptor; HIgR; Nectin-1; CD111; PVRL1; HVEC; PRR1

UniProt

Q15223

Expression Region

Gln31-Thr334

Conjugation

Unconjugated

Tag

C-6His

Origin

Human

Field of Research

Neuroscience

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)

Bioactivity

BLI: Loaded Human Nectin-4-mFc (Cat#orb2993807) on AMC Biosensor, can bind Human Nectin-1-His (Cat#orb2995040) with an affinity constant of 27.7 nM as determined in BLI assay. Loaded Human Nectin-4-Fc (Cat#orb2993880) on Protein A Biosensor, can bind Human Nectin-1-His (Cat#orb2995040) with an affinity constant of 24.18 nM as determined in BLI assay. Loaded Biotinylated Human Nectin-4-His-Avi (Cat#orb2993794) on SA Biosensor, can bind Human Nectin-1-His (Cat#orb2995040) with an affinity constant of 8.83 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 34.99 KDa. Observed: 40-60 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

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