Recombinant Human CD19 Protein
Recombinant Human CD19 is produced by our Mammalian expression system and the target gene encoding Pro20-Lys291 is expressed with a human IgG1 Fc tag at the C-terminus.
Product Specifications
Product Name Alternative
B-Lymphocyte Antigen CD19; B-Lymphocyte Surface Antigen B4; Differentiation Antigen CD19; T-Cell Surface Antigen Leu-12; CD19
UniProt
P15391
Expression Region
Pro20-Lys291
Conjugation
Unconjugated
Tag
C-Fc
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Bioactivity
ELISA: Immobilized Human FMC63 at 2μg/ml (100 μl/well) can bind Human CD19-Fc (Cat#orb2994989) . The ED50 of Recombinant Human CD19-Fc (Cat#orb2994989) is 55.28 ng/ml.. BLI: Loaded Anti-Human CD19 mAb-Fc (Cat#orb2993102) on AMC Biosensor, can bind Human CD19-FC (Cat#orb2994989) with an affinity constant of 2.72 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 57.3 KDa. Observed: 80-95 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Available Sizes
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