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Recombinant Human DKK-1 Protein

Recombinant Human Dickkopf-related Protein 1 is produced by our Mammalian expression system and the target gene encoding Thr32-His266 is expressed with a 8His tag at the N-terminus.

Product Specifications

Product Name Alternative

Dickkopf-related protein 1; Dickkopf-1; Dkk-1

UniProt

O94907

Expression Region

Thr32-His266

Conjugation

Unconjugated

Tag

N-8His

Origin

Human

Field of Research

Stem Cell & Developmental Biology

Endotoxin

< 0.01 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Human DKK-1-His (Cat#orb2994936) at 2μg/ml (100 μl/well) can bind Anti-Human DKK-1 mAb (Cat#orb2993101) . The ED50 of Anti-Human DKK-1 mAb (Cat#orb2993101) is 5.57 ng/ml (Regularly tested) .. BLI: Loaded Anti-Human DKK-1 mAb-Fc (Cat#orb2993101) on Protein A Biosensor, can bind Human DKK-1-His (Cat#orb2994936) with an affinity constant of 1.59 pM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 27 KDa. Observed: 35-50 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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