Recombinant Human DKK-1 Protein
Recombinant Human Dickkopf-related Protein 1 is produced by our Mammalian expression system and the target gene encoding Thr32-His266 is expressed with a 8His tag at the N-terminus.
Product Specifications
Product Name Alternative
Dickkopf-related protein 1; Dickkopf-1; Dkk-1
UniProt
O94907
Expression Region
Thr32-His266
Conjugation
Unconjugated
Tag
N-8His
Origin
Human
Field of Research
Stem Cell & Developmental Biology
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human DKK-1-His (Cat#orb2994936) at 2μg/ml (100 μl/well) can bind Anti-Human DKK-1 mAb (Cat#orb2993101) . The ED50 of Anti-Human DKK-1 mAb (Cat#orb2993101) is 5.57 ng/ml (Regularly tested) .. BLI: Loaded Anti-Human DKK-1 mAb-Fc (Cat#orb2993101) on Protein A Biosensor, can bind Human DKK-1-His (Cat#orb2994936) with an affinity constant of 1.59 pM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 27 KDa. Observed: 35-50 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
