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Recombinant Human VEGF 121 Protein

Recombinant Human Vascular Endothelial Growth Factor A is produced by our E.coli expression system and the target gene encoding Ala27-Arg147 is expressed.

Product Specifications

Product Name Alternative

Vascular endothelial growth factor A; VEGFA; VEGF-A; Vascular permeability factor; VPF; VEGF; VEGF121

UniProt

P15692

Expression Region

Ala27-Arg147

Conjugation

Unconjugated

Tag

Tag free

Origin

Human

Field of Research

Cardiovascular Research; Cell Biology

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Human VEGF 121 (Cat#orb2994925) at 2μg/ml (100 μl/well) can bind Human VEGFR2-Fc (Cat#orb2994455) .The ED50 of Human VEGFR2-Fc (Cat#orb2994455) is 13.04 ng/ml.. BLI: Loaded Human VEGF Antibody (5E7) -Fc (Cat#DA082) on AHC Biosensor, can bind Human VEGF 121 (Cat#orb2994925) with an affinity constant of 1.31 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 14.2 KDa. Observed: 16 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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