Recombinant Human VEGF 121 Protein
Recombinant Human Vascular Endothelial Growth Factor A is produced by our E.coli expression system and the target gene encoding Ala27-Arg147 is expressed.
Product Specifications
Product Name Alternative
Vascular endothelial growth factor A; VEGFA; VEGF-A; Vascular permeability factor; VPF; VEGF; VEGF121
UniProt
P15692
Expression Region
Ala27-Arg147
Conjugation
Unconjugated
Tag
Tag free
Origin
Human
Field of Research
Cardiovascular Research; Cell Biology
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human VEGF 121 (Cat#orb2994925) at 2μg/ml (100 μl/well) can bind Human VEGFR2-Fc (Cat#orb2994455) .The ED50 of Human VEGFR2-Fc (Cat#orb2994455) is 13.04 ng/ml.. BLI: Loaded Human VEGF Antibody (5E7) -Fc (Cat#DA082) on AHC Biosensor, can bind Human VEGF 121 (Cat#orb2994925) with an affinity constant of 1.31 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 14.2 KDa. Observed: 16 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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