Recombinant Human CD40L Protein

CD40 Ligand; CD40-L; T-Cell Antigen Gp39; TNF-Related Activation Protein; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP

ELISA: Immobilized Recombinant Human CD40 (C-Fc) (Cat#orb2994010) at 2μg/ml (100 μl/well) can bind Recombinant Human CD40L (Cat#orb2995248) *.*: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Human CD40L (Cat#orb2995248) is 1-10 ng/ml. (QC verified) . BLI: Loaded Human CD40-His (Cat#orb2995146) on HIS1K Biosensor, can bind Human CD40L (Cat#orb2995248) with an affinity constant of 2.43 nM as determined in BLI assay. (Regularly tested) Loaded Anti-Human CD40L mAb-Fc (Cat#orb2993151) on Protein A Biosensor, can bind Human CD40L (Cat#orb2995248) with an affinity constant of 0.05 nM as determined in BLI assay.

Huey-Liang Kuo, Chiu-Ching Huang, Tze-Yi Lin, Ching-Yuang Lin IL-17 and CD40 ligand synergistically stimulate the chronicity of diabetic nephropathy Nephrology Dialysis Transplantation, 33, 248 (2018)

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.