Recombinant Human IL-17A Protein
Recombinant Human Interleukin-17A is produced by our E.coli expression system and the target gene encoding Gly24-Ala155 is expressed.
Product Specifications
Product Name Alternative
Interleukin-17A; IL-17; IL-17A; Cytotoxic T-Lymphocyte-Associated Antigen 8; CTLA-8; IL17A; CTLA8; IL17
UniProt
Q16552
Expression Region
Gly24-Ala155
Conjugation
Unconjugated
Tag
Tag free
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human IL-17A (Cat#orb2995247) at 2μg/ml (100 μl/well) can bind Anti-Human IL-17A mAb (Cat#orb2993149) .The ED50 of Anti-Human IL-17A mAb (Cat#orb2993149) is 0.185 ug/ml.. BLI: Loaded Human IL-17RA-Fc (Cat#orb2994535) on Protein A Biosensor, can bind Human IL-17A (Cat#orb2995247) with an affinity constant of -3 nM as determined in BLI assay. Loaded Biotinylated Human IL-17RA-His-Avi (Cat#orb2993686) on HIS1K Biosensor, can bind Human IL-17A (Cat#orb2995247) with an affinity constant of 0.50 nM as determined in BLI assay. Loaded Biotinylated Human IL-17RA-Fc (Cat#orb2993676) on Pro A Biosensor, can bind Human IL-17A (Cat#orb2995247) with an affinity constant of 0.79 nM as determined in BLI assay.. Cell Based Assay: Measured by its ability to induce IL-6 secretion by NIH‐3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 1-7 ng/ml.
Form
Lyophilized
Molecular Weight
Predicted: 15.26 KDa. Observed: 16 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Available Sizes
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