Recombinant Human IL-17F Protein
Recombinant Human Interleukin-17F is produced by our E.coli expression system and the target gene encoding Arg31-Gln163 is expressed.
Product Specifications
Product Name Alternative
Interleukin-17F; IL-17F; Cytokine ML-1; IL17F
UniProt
Q96PD4
Expression Region
Arg31-Gln163
Conjugation
Unconjugated
Tag
Tag free
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human IL-17F (Cat#orb2995245) at 2μg/ml (100 μl/well) can bind Biotinylated Human IL-17RA-His-Avi (Cat#orb2993686) .The ED50 of Biotinylated Human IL-17RA-His-Avi (Cat#orb2993686) is 0.11 ug/ml.. BLI: Loaded Biotinylated Human IL-17RA-His-Avi (Cat#orb2993686) on HIS1K Biosensor, can bind Human IL-17F (Cat#orb2995245) with an affinity constant of 0.56 uM nM as determined in BLI assay. Loaded Biotinylated Human IL-17RA-Fc (Cat#orb2993676) on Pro A Biosensor, can bind Human IL-17F (Cat#orb2995245) with an affinity constant of 0.15uM as determined in BLI assay.. Cell Based Assay: Measured by its ability to induce IL-6 secretion by NIH‐3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 5-40 ng/ml.
Form
Lyophilized
Molecular Weight
Predicted: 15 KDa. Observed: 15 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, pH 7.4.
Available Sizes
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