Recombinant Human IL-17F Protein

Recombinant Human Interleukin-17F is produced by our E.coli expression system and the target gene encoding Arg31-Gln163 is expressed.

Product Specifications

Product Name Alternative

Interleukin-17F; IL-17F; Cytokine ML-1; IL17F

UniProt

Q96PD4

Expression Region

Arg31-Gln163

Conjugation

Unconjugated

Tag

Tag free

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Human IL-17F (Cat#orb2995245) at 2μg/ml (100 μl/well) can bind Biotinylated Human IL-17RA-His-Avi (Cat#orb2993686) .The ED50 of Biotinylated Human IL-17RA-His-Avi (Cat#orb2993686) is 0.11 ug/ml.. BLI: Loaded Biotinylated Human IL-17RA-His-Avi (Cat#orb2993686) on HIS1K Biosensor, can bind Human IL-17F (Cat#orb2995245) with an affinity constant of 0.56 uM nM as determined in BLI assay. Loaded Biotinylated Human IL-17RA-Fc (Cat#orb2993676) on Pro A Biosensor, can bind Human IL-17F (Cat#orb2995245) with an affinity constant of 0.15uM as determined in BLI assay.. Cell Based Assay: Measured by its ability to induce IL-6 secretion by NIH‐3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 5-40 ng/ml.

Form

Lyophilized

Molecular Weight

Predicted: 15 KDa. Observed: 15 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.