Recombinant Human ST2 Protein

Recombinant Human Interleukin-1 Receptor-like 1 is produced by our Mammalian expression system and the target gene encoding Lys19-Phe328 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Interleukin-1 receptor-like 1; Protein ST2; DER4; ST2; T1

UniProt

Q01638

Expression Region

Lys19-Phe328

Conjugation

Unconjugated

Tag

C-6His

Origin

Human

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Human ST2-His (Cat#orb2994520) at 5μg/ml (100 μl/well) can bind Human IL-33-His (Cat#C233) .The ED50 of Human IL-33-His (Cat#C233) is 8.44 ng/ml.. BLI: Loaded Human IL-33 (Cat#orb2995234) on AR2G Biosensor, can bind Human ST2-His (Cat#orb2994520) with an affinity constant of 0.97nM as determined in BLI assay. Loaded Human sST2 Antibody-mFc (Cat#DA014) on AMC Biosensor, can bind Human ST2-His (Cat#orb2994520) with an affinity constant of -3 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 36 KDa. Observed: 50-85 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.