Recombinant Human 4-1BB Protein
Recombinant Human 4-1BB Ligand Receptor is produced by our Mammalian expression system and the target gene encoding Leu24-Gln186 is expressed with a human IgG1 Fc, 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
CD137; ILA; TNFRSF9; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA
UniProt
Q07011
Expression Region
Leu24-Gln186
Conjugation
Unconjugated
Tag
C-Fc-6His
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human 4-1BB-Fc-His (Cat#orb2994490) at 2μg/ml (100 μl/well) can bind Anti-Human CD137 mAb (Cat#orb2993114) .The ED50 of Anti-Human CD137 mAb (Cat#orb2993114) is 0.41ug/ml.. BLI: Loaded Human 4-1BB-Fc-His (Cat#orb2994490) on HIS1K Biosensor, can bind Anti-Human CD137 mAb (Cat#orb2993114) with an affinity constant of 12.2 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 44 KDa. Observed: 55-75 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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