Recombinant Human VEGFR2 Protein
Recombinant Human Vascular Endothelial Growth Factor Receptor 2 is produced by our Mammalian expression system and the target gene encoding Ala20-Glu764 is expressed with a human IgG1 Fc tag at the C-terminus.
Product Specifications
Product Name Alternative
Vascular endothelial growth factor receptor 2; KDR; VEGFR-2; Fetal liver kinase 1; FLK-1; Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1
UniProt
P35968
Expression Region
Ala20-Glu764
Conjugation
Unconjugated
Tag
C-Fc
Origin
Human
Field of Research
Cardiovascular Research; Cell Biology
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human VEGF121-His (Cat#orb2994931) at 10μg/ml (100 μl/well) can bind Human VEGFR2-Fc (Cat#orb2994455) . The ED50 of Recombinant Human VEGFR2-Fc (Cat#orb2994455) is 1.92 ug/ml. Immobilized Human VEGF 121 (Cat#orb2994925) at 2μg/ml (100 μl/well) can bind Human VEGFR2-Fc (Cat#orb2994455) .The ED50 of Human VEGFR2-Fc (Cat#orb2994455) is 13.04 ng/ml.
Form
Lyophilized
Molecular Weight
Predicted: 110.4 KDa. Observed: 150-160 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20 mM Tris-HCl, 15% Maltose, 0.05% Tween 80, pH 9.0.
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