Recombinant Human VEGFR1 Protein
Recombinant Human Vascular Endothelial Growth Factor Receptor 1 is produced by our Mammalian expression system and the target gene encoding Ser27-Asn756 is expressed with a human IgG1 Fc tag at the C-terminus.
Product Specifications
Product Name Alternative
Vascular endothelial growth factor receptor 1; VEGFR-1; Fms-like tyrosine kinase 1; FLT-1; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; Vascular permeability factor receptor
UniProt
P17948
Expression Region
Ser27-Asn756
Conjugation
Unconjugated
Tag
C-Fc
Origin
Human
Field of Research
Cardiovascular Research
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human VEGF165 (Cat#orb2995236) at 10μg/ml (100 μl/well) can bind Human VEGFR1-Fc (Cat#orb2994454) . The ED50 of Recombinant Human VEGFR1-Fc (Cat#orb2994454) is 36.4 ng/ml.. BLI: Loaded Biotinylated Human VEGF165 Protein (C-6His-Avi) (Cat#orb2992964) on HIS1K Biosensor, can bind Recombinant Human VEGFR1 (C-Fc) (Cat#orb2994454) with an affinity constant of 10.2 pM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 109.3 KDa. Observed: 150-190 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5%Trehalose, 5%Mannitol, 0.01%Tween80, pH 7.0.
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