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Recombinant Rat VEGF 164 Protein

Recombinant Rat Vascular Endothelial Growth Factor A is produced by our Yeast expression system and the target gene encoding Ala27-Arg190 (Ala36Thr) is expressed.

Product Specifications

Product Name Alternative

Vascular endothelial growth factor A; Vascular permeability factor; VEGF; VEGF-A; VPF

UniProt

P16612

Expression Region

Ala27-Arg190 (Ala36Thr)

Conjugation

Unconjugated

Tag

Tag free

Origin

Rat

Field of Research

Cardiovascular Research; Cell Biology

Endotoxin

< 0.1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Rat VEGF 164 (Cat#orb2994452) at 2μg/ml (100 μl/well) can bind Mouse VEGFR2-Fc (Cat#orb2993886) .The ED50 of Mouse VEGFR2-Fc (Cat#orb2993886) is 22.17 ng/ml (Regularly tested) .. BLI: Loaded Mouse VEGFR2-Fc (Cat#orb2993886) on Protein A Biosensor, can bind Rat VEGF 164 (Cat#orb2994452) with an affinity constant of 0.26 nM as determined in BLI assay. Loaded Human VEGF Antibody (5E7) -Fc (Cat#DA082) on AHC Biosensor, can bind Rat VEGF 164 (Cat#orb2994452) with an affinity constant of 1.09 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 19.2 KDa. Observed: 18-23 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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