Recombinant Rat VEGF 164 Protein
Recombinant Rat Vascular Endothelial Growth Factor A is produced by our Yeast expression system and the target gene encoding Ala27-Arg190 (Ala36Thr) is expressed.
Product Specifications
Product Name Alternative
Vascular endothelial growth factor A; Vascular permeability factor; VEGF; VEGF-A; VPF
UniProt
P16612
Expression Region
Ala27-Arg190 (Ala36Thr)
Conjugation
Unconjugated
Tag
Tag free
Origin
Rat
Field of Research
Cardiovascular Research; Cell Biology
Endotoxin
< 0.1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Rat VEGF 164 (Cat#orb2994452) at 2μg/ml (100 μl/well) can bind Mouse VEGFR2-Fc (Cat#orb2993886) .The ED50 of Mouse VEGFR2-Fc (Cat#orb2993886) is 22.17 ng/ml (Regularly tested) .. BLI: Loaded Mouse VEGFR2-Fc (Cat#orb2993886) on Protein A Biosensor, can bind Rat VEGF 164 (Cat#orb2994452) with an affinity constant of 0.26 nM as determined in BLI assay. Loaded Human VEGF Antibody (5E7) -Fc (Cat#DA082) on AHC Biosensor, can bind Rat VEGF 164 (Cat#orb2994452) with an affinity constant of 1.09 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 19.2 KDa. Observed: 18-23 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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