Recombinant Human B7-H3 Protein
Recombinant Human B7 Homolog 3 is produced by our Mammalian expression system and the target gene encoding Leu29-Thr461 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
CD276; B7H34Ig-B7-H3; B7-H3; B7 homolog 3; CD276 antigen; CD276 molecule; Costimulatory molecule
UniProt
Q5ZPR3
Expression Region
Leu29-Thr461
Conjugation
Unconjugated
Tag
C-6His
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human B7-H3-His (Cat#orb2994418) at 2μg/ml (100 μl/well) can bind Anti-Human B7-H3 mAb (Cat#orb2993104) . The ED50 of Anti-Human B7-H3 mAb (Cat#orb2993104) is 7.98 ng/ml. (Regularly tested) . BLI: Loaded Anti-Human B7-H3 mAb-Fc (Cat#orb2993104) on Protein A Biosensor, can bind Human B7-H3-His (Cat#orb2994418) with an affinity constant of 0.7 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 47.3 KDa. Observed: 65-90 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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