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Recombinant Mouse ST2 Protein

Recombinant Mouse Interleukin-1 receptor-like 1 is produced by our Mammalian expression system and the target gene encoding Ser27-Ala337 (Val192Ala) is expressed with a human IgG1 Fc tag at the C-terminus.

Product Specifications

Product Name Alternative

Interleukin-1 receptor-like 1; Lymphocyte antigen 84; Protein ST2; Protein T1; Il1rl1; DER4; Fit-1; IL-1 R4; IL-1RL1; IL-33R; Ly84; ST2L; ST2V; suppression of tumorigenicity 2

UniProt

P14719

Expression Region

Ser27-Ala337 (Val192Ala)

Conjugation

Unconjugated

Tag

C-Fc

Origin

Mouse

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Mouse IL-33 (Cat#orb2994601) at 5μg/ml (100 μl/well) can bind Mouse ST2-Fc (Cat#orb2994391) .The ED50 of Mouse ST2-Fc (Cat#orb2994391) is 0.25 ug/ml.. BLI: Loaded Human IL-33 (Cat#orb2995234) on AR2G Biosensor, can bind Mouse ST2-Fc (Cat#orb2994391) with an affinity constant of 2.28nM as determined in BLI assay. Loaded Mouse IL-33 (Cat#orb2994601) on AR2G Biosensor, can bind Mouse ST2-Fc (Cat#orb2994391) with an affinity constant of 1.49M as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 62.7 KDa. Observed: 90-110 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

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