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Recombinant Human PD-L1 Protein

Recombinant Human Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a Flag tag at the C-terminus.

Product Specifications

Product Name Alternative

Programmed Cell Death 1 Ligand 1; PD-L1; PDCD1 Ligand 1; Programmed Death Ligand 1; B7 Homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1

UniProt

Q9NZQ7

Expression Region

Phe19-Thr239

Conjugation

Unconjugated

Tag

C-Flag

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Human PD-L1-Flag (Cat#orb2994368) at 2μg/ml (100 μl/well) can bind Anti-Human PDL1 mAb-Fc (Cat#orb2993144) .The ED50 of Anti-Human PDL1 mAb-Fc (Cat#orb2993144) is 14.68 ng/ml.. BLI: Loaded Human PD-1-Fc (Cat#orb2994333) on Protein A Biosensor, can bind Human PD-L1-Flag (Cat#orb2994368) with an affinity constant of 0.19 uM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 26.3 KDa. Observed: 35-40 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Available Sizes

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