Recombinant Human PD-L1 Protein
Recombinant Human Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a Flag tag at the C-terminus.
Product Specifications
Product Name Alternative
Programmed Cell Death 1 Ligand 1; PD-L1; PDCD1 Ligand 1; Programmed Death Ligand 1; B7 Homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1
UniProt
Q9NZQ7
Expression Region
Phe19-Thr239
Conjugation
Unconjugated
Tag
C-Flag
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human PD-L1-Flag (Cat#orb2994368) at 2μg/ml (100 μl/well) can bind Anti-Human PDL1 mAb-Fc (Cat#orb2993144) .The ED50 of Anti-Human PDL1 mAb-Fc (Cat#orb2993144) is 14.68 ng/ml.. BLI: Loaded Human PD-1-Fc (Cat#orb2994333) on Protein A Biosensor, can bind Human PD-L1-Flag (Cat#orb2994368) with an affinity constant of 0.19 uM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 26.3 KDa. Observed: 35-40 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Available Sizes
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