Recombinant Human LIGHT Protein
Recombinant Human TNF Ligand Superfamily Member 14 is produced by our Mammalian expression system and the target gene encoding Leu83-Val240 is expressed with a 6His tag at the N-terminus.
Product Specifications
Product Name Alternative
Tumor necrosis factor ligand superfamily member 14; Herpes virus entry mediator ligand; TNFSF14; HVEM-L; LIGHT
Expression Region
Leu83-Val240
Conjugation
Unconjugated
Tag
N-6His
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Recombinant Human LIGHT (N-6His) (Cat#orb2994330) at 5μg/ml (100 μl/well) can bind Recombinant Rhesus Macaque HVEM (C-Fc) (Cat#orb2994821) .The ED50 of Recombinant Rhesus Macaque HVEM (C-Fc) (Cat#orb2994821) is 17.51 ng/ml. (Regularly tested) . BLI: Loaded Mouse LTBR-Fc (Cat#orb2993964) on Protein A Biosensor, can bind Human LIGHT-His (Cat#orb2994330) with an affinity constant of 0.81 nM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 18.2 KDa. Observed: 18-24 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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