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Recombinant Human LIGHT Protein

Recombinant Human TNF Ligand Superfamily Member 14 is produced by our Mammalian expression system and the target gene encoding Leu83-Val240 is expressed with a 6His tag at the N-terminus.

Product Specifications

Product Name Alternative

Tumor necrosis factor ligand superfamily member 14; Herpes virus entry mediator ligand; TNFSF14; HVEM-L; LIGHT

Expression Region

Leu83-Val240

Conjugation

Unconjugated

Tag

N-6His

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Recombinant Human LIGHT (N-6His) (Cat#orb2994330) at 5μg/ml (100 μl/well) can bind Recombinant Rhesus Macaque HVEM (C-Fc) (Cat#orb2994821) .The ED50 of Recombinant Rhesus Macaque HVEM (C-Fc) (Cat#orb2994821) is 17.51 ng/ml. (Regularly tested) . BLI: Loaded Mouse LTBR-Fc (Cat#orb2993964) on Protein A Biosensor, can bind Human LIGHT-His (Cat#orb2994330) with an affinity constant of 0.81 nM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 18.2 KDa. Observed: 18-24 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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