Recombinant Human CD40L Protein
Recombinant Human CD40 Ligand is produced by our Mammalian expression system and the target gene encoding Met113-Leu261 is expressed with a mFc tag at the N-terminus.
Product Specifications
Product Name Alternative
CD40 Ligand; CD40-L; T-Cell Antigen Gp39; TNF-Related Activation Protein; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP
UniProt
P29965
Expression Region
Met113-Leu261
Conjugation
Unconjugated
Tag
N-mFc
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Bioactivity
BLI: Loaded Anti-Human CD40L mAb-Fc (Cat#orb2993151) on AHC Biosensor, can bind Human CD40L-mFc (Cat#orb2993921) with an affinity constant of -3 nM as determined in BLI assay. (Regularly tested) Loaded Human CD40-His (Cat#orb2995146) on HIS1K Biosensor, can bind Human CD40L-mFc (Cat#orb2993921) with an affinity constant of 4.21 nM as determined in BLI assay. Loaded Cynomolgus CD40-His (Cat#orb2994787) on HIS1K Biosensor, can bind Human CD40L-mFc (Cat#orb2993921) with an affinity constant of 3.62 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 42.7 KDa. Observed: 50&90 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, 1mM EDTA, pH 7.4.
Available Sizes
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