Recombinant Human CD3E Protein
Recombinant Human T-Cell Surface Glycoprotein CD3 Epsilon Chain is produced by our Mammalian expression system and the target gene encoding Gln22-Thr48 is expressed with a mouse IgG1 Fc tag at the C-terminus.
Product Specifications
Product Name Alternative
T-Cell Surface Glycoprotein CD3 Epsilon Chain; T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e; CD3E; T3E
UniProt
P07766
Expression Region
Gln22-Thr48
Conjugation
Unconjugated
Tag
C-mFc
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Anti-Human/Monkey CD3E mAb (Cat#orb2993159) at 2μg/ml (100 μl/well) can bind Human CD3E-mFc (Cat#orb2993920) .The ED50 of Human CD3E-mFc (Cat#orb2993920) is 28.99 ng/ml.. BLI: Loaded Anti-Human/Monkey CD3E mAb-Fc (Cat#orb2993159) on AHC Biosensor, can bind Human CD3E-mFc (Cat#orb2993920) with an affinity constant of -3 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 28.5 KDa. Observed: 32-40 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
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