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Recombinant Human IL-23 Protein

Recombinant Human Interleukin-23 is produced by our Mammalian expression system and the target gene encoding Ile23-Ser328&Ala21-Pro189 is expressed with a human IgG1 Fc tag at the C-terminus.

Product Specifications

Product Name Alternative

SGRF; IL-23p19; CLMF p40; IL-12 subunit p40; NKSF2

UniProt

Q9NPF7, P29460

Expression Region

Ile23-Ser328&Ala21-Pro189

Conjugation

Unconjugated

Tag

C-Fc

Origin

Human

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Human IL-23R-His (Cat#orb2994303) at 5μg/ml (100 μl/well) can bind Human IL-23-Fc (Cat#orb2993914) .The ED50 of Human IL-23-Fc (Cat#orb2993914) is 0.187 ug/ml.. Cell Based Assay: Measured by its ability to induce STAT reporter activity in 293F human embryonic kidney cells. The ED50 for this effect is 179.42 ng/ml.

Form

Lyophilized

Molecular Weight

Predicted: 81.3 KDa. Observed: 80-110 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

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