Recombinant Human IL-23 Protein

ELISA: Immobilized Human IL-23R-Fc (Cat#orb2994313) at 10μg/ml (100 μl/well) can bind Human IL-23-His (Cat#orb2993911) .The ED50 of Human IL-23-His (Cat#orb2993911) is 0.248 ug/ml.. BLI: Loaded Ustekinumab on Pro-A Biosensor, can bind Recombinant Human IL-23 (C-6His) (Cat#orb2993911) with an affinity constant of 40.7 pM as determined in BLI assay. (Regularly tested) Loaded Human IL-23R-Fc (Cat#orb2994313) on Protein A Biosensor, can bind Human IL-23-His (Cat#orb2993911) with an affinity constant of 0.98 nM as determined in BLI assay. Loaded Anti-Human IL23-P19 mAb-Fc (Cat#orb2993116) on Protein A Biosensor, can bind Human IL-23-His (Cat#orb2993911) with an affinity constant of 2.2 pM as determined in BLI assay. . Cell Based Assay: Measured by its ability to induce STAT reporter activity in 293F human embryonic kidney cells. The ED50 for this effect is 70-210 ng/ml. (QC verified)

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.