Recombinant Human GPC3 Protein

Recombinant Human Glypican-3 is produced by our Mammalian expression system and the target gene encoding Gln25-His559 is expressed with a human IgG1 Fc tag at the C-terminus.

Product Specifications

Product Name Alternative

Glypican-3; GTR2-2; Intestinal protein OCI-5; MXR7; GPC3; OCI5

UniProt

P51654

Expression Region

Gln25-His559

Conjugation

Unconjugated

Tag

C-Fc

Origin

Human

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.

Bioactivity

BLI: Loaded Human FGFb (157AA) (Cat#orb3002481) on APS Biosensor, can bind Human GPC3-Fc (Cat#orb2993899) with an affinity constant of 0.7 nM as determined in BLI assay. (Regularly tested) Loaded Human FGFb (155AA) (Cat#orb3002364) on APS Biosensor, can bind Human GPC3-Fc (Cat#orb2993899) with an affinity constant of 0.68 nM as determined in BLI assay. (Regularly tested) Loaded Human FGFb (146AA) (Cat#orb3002475) on APS Biosensor, can bind Human GPC3-Fc (Cat#orb2993899) with an affinity constant of 0.6 nM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 87.7 KDa. Observed: 40 KDa & 65 KDa & 90-250 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.