Recombinant Cynomolgus TSLP Protein
Recombinant Cynomolgus Thymic Stromal Lymphopoietin is produced by our E.coli expression system and the target gene encoding Tyr29-Gln159 (Glu37Gln) is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Thymic stromal lymphopoietin; Thymic stroma-derived lymphopoietin; Tslp
Expression Region
Tyr29-Gln159 (Glu37Gln)
Conjugation
Unconjugated
Tag
C-6His
Origin
Cynomolgus
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Anti-Human TSLP mAb (Cat#orb2992721) at 2μg/ml (100 μl/well) can bind Cynomolgus TSLP-His (Cat#orb2993896) . The ED50 of Cynomolgus TSLP-His (Cat#orb2993896) is 4.48 ng/ml.. BLI: Loaded Anti-Human TSLP mAb-Fc (Cat#orb2992721) on Protein A Biosensor, can bind Cynomolgus TSLP-His (Cat#orb2993896) with an affinity constant of 0.07 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 16.2 KDa. Observed: 16 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM Tris-HCl, 6% Trehalose, 2% Glycine, 50mM NaCl, 0.05% Tween 80, pH 7.5.
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