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Recombinant Human TPBG Protein

Recombinant Human Trophoblast glycoprotein is produced by our Mammalian expression system and the target gene encoding Ser32-Ser355 is expressed with a mouse IgG1 Fc tag at the C-terminus.

Product Specifications

Product Name Alternative

5T4 Protein; 5T4AG Protein; M6P1 Protein; WAIF1 Protein; TPBG

UniProt

Q13641

Expression Region

Ser32-Ser355

Conjugation

Unconjugated

Tag

C-mFc

Origin

Human

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Human TPBG (C-mFc) (Cat#orb2993866) at 2 μg/ml (100 μl/well) can bind Human TPBG Antibody (5G9) (Human IgG1) (Cat#orb2992640) . The ED50 of Human TPBG Antibody (5G9) (Human IgG1) (Cat#orb2992640) is 3.02 ng/ml. (Regularly tested) . BLI: Loaded Human TPBG Antibody (5G9) (Human IgG1) (Cat#orb2992640) on Protein-A Biosensor, can bind Human TPBG (C-mFc) (Cat#orb2993866) with an affinity constant of 8.75 nM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 61.7 KDa. Observed: 80-110 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

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