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Recombinant Cynomolgus Nectin-4 Protein

Recombinant Cynomolgus Poliovirus Receptor-Related Protein 4 is produced by our Mammalian expression system and the target gene encoding Gly32-Ser349 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

PVRL4; Nectin-4; Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4; PRR4; LNIR

UniProt

L0N6D9

Expression Region

Gly32-Ser349

Conjugation

Unconjugated

Tag

C-6His

Origin

Cynomolgus

Field of Research

Cancer Biology

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Anti-Human Nectin-4 mAb-mFc (Cat#orb2993141) at 2μg/ml (100 μl/well) can bind Cynomolgus Nectin-4-His (Cat#orb2993865) . The ED50 of Cynomolgus Nectin-4 -His (Cat#orb2993865) is 36.5 ng/ml. (Regularly tested) . BLI: Loaded Anti-Human Nectin-4 mAb-mFc (Cat#orb2993141) on AMQ Biosensor, can bind Cynomolgus Nectin-4-His (Cat#orb2993865) with an affinity constant of 1.57 nM as determined in BLI assay.

Form

Lyophilized

Molecular Weight

Predicted: 34.9 KDa. Observed: 38-50 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Available Sizes

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