Recombinant Cynomolgus Nectin-4 Protein
Recombinant Cynomolgus Poliovirus Receptor-Related Protein 4 is produced by our Mammalian expression system and the target gene encoding Gly32-Ser349 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
PVRL4; Nectin-4; Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4; PRR4; LNIR
UniProt
L0N6D9
Expression Region
Gly32-Ser349
Conjugation
Unconjugated
Tag
C-6His
Origin
Cynomolgus
Field of Research
Cancer Biology
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Anti-Human Nectin-4 mAb-mFc (Cat#orb2993141) at 2μg/ml (100 μl/well) can bind Cynomolgus Nectin-4-His (Cat#orb2993865) . The ED50 of Cynomolgus Nectin-4 -His (Cat#orb2993865) is 36.5 ng/ml. (Regularly tested) . BLI: Loaded Anti-Human Nectin-4 mAb-mFc (Cat#orb2993141) on AMQ Biosensor, can bind Cynomolgus Nectin-4-His (Cat#orb2993865) with an affinity constant of 1.57 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 34.9 KDa. Observed: 38-50 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
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