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Recombinant Mouse LEPR Protein

Recombinant Mouse Leptin Receptor is produced by our Mammalian expression system and the target gene encoding Leu22-Gly839 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Leptin receptor; LEP-R; HuB219; OB receptor; OB-R; CD295; LEPR; DB; OBR

UniProt

P48356

Expression Region

Leu22-Gly839

Conjugation

Unconjugated

Tag

C-10His

Origin

Mouse

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. (QC verified)

Bioactivity

ELISA: Immobilized Recombinant Mouse LEPR (C-10His) (Cat#orb2993840) at 5μg/ml (100 μl/well) can bind Recombinant Mouse Leptin (Cat#orb3002378) *. *: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Mouse Leptin (Cat#orb3002378) is 1.16 ng/ml. (Regularly tested) .. BLI: Loaded Recombinant Mouse LEPR (C-10His) (Cat#orb2993840) on HIS1K Biosensor, can bind Recombinant Mouse Leptin (Cat#orb3002378) with an affinity constant of 0.196 nM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 93 KDa. Observed: 120-150 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of PBS, pH 7.4.

Available Sizes

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