Recombinant Mouse LEPR Protein
Recombinant Mouse Leptin Receptor is produced by our Mammalian expression system and the target gene encoding Leu22-Gly839 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Leptin receptor; LEP-R; HuB219; OB receptor; OB-R; CD295; LEPR; DB; OBR
UniProt
P48356
Expression Region
Leu22-Gly839
Conjugation
Unconjugated
Tag
C-10His
Origin
Mouse
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
Bioactivity
ELISA: Immobilized Recombinant Mouse LEPR (C-10His) (Cat#orb2993840) at 5μg/ml (100 μl/well) can bind Recombinant Mouse Leptin (Cat#orb3002378) *. *: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Mouse Leptin (Cat#orb3002378) is 1.16 ng/ml. (Regularly tested) .. BLI: Loaded Recombinant Mouse LEPR (C-10His) (Cat#orb2993840) on HIS1K Biosensor, can bind Recombinant Mouse Leptin (Cat#orb3002378) with an affinity constant of 0.196 nM as determined in BLI assay. (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 93 KDa. Observed: 120-150 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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